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本文报道免疫沉淀法定量测定GOT同工酶的方法,此法系在改良Reitman法基础上增加免疫沉淀程序,便于广大临床实验室应用。55名正常人GOTs为18±5U、GOT_m为2.2±2.8U。12名急性肝炎、18名慢活肝和9名肝癌与正常人比较GOT_m值差异非常显著(p<0.01),二种GOT同工酶测定为从血清内了解肝实质细胞结构性损伤的程度提供有效参数,对疾病监护和预后具有一定价值。对慢话肝和慢迁肝的鉴别是否能提供较为可靠的依据,有待进一步探讨。
This article reports the immunoprecipitation method for the quantitative determination of GOT isoenzymes, this method is based on the improved Reitman method to increase the immunoprecipitation program for the general clinical laboratory applications. 55 normal GOTs 18 ± 5U, GOT_m 2.2 ± 2.8U. There was a significant difference in GOT_m values between 12 acute hepatitis, 18 slow-living and 9 liver cancers (p <0.01), and two GOT isoenzymes were measured to understand the extent of structural damage of hepatic parenchymal cells from the serum Effective parameters, the disease monitoring and prognosis of a certain value. The identification of dysthymia and slow-moving liver can provide a more reliable basis to be further explored.