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目的:研究塞来昔布对前列腺癌DU-145细胞凋亡及侵袭力的影响,并探讨其可能作用机制。方法:应用Hoechst 33342/PI染色检测细胞凋亡形态;Annexin V-FITC/PI双染色流式细胞术检测不同浓度塞来昔布诱导细胞凋亡能力;RT-PCR法检测塞来昔布作用后Bcl-2、E-cadherin、ICE及COX-2 mRNA表达水平的变化。结果:Hoechst 33342/PI双染色可观察到药物作用后,细胞呈现明显凋亡现象。流式细胞术证实塞来昔布能有效诱导细胞凋亡,0、25、50、100、200μmol/L塞来昔布诱导细胞凋亡率分别为(1.10±0.15)%,(3.87±0.79)%,(10.59±1.58)%,(22.50±3.30)%,(33.85±2.71)%,细胞凋亡率呈现浓度依赖性递增,RT-PCR显示Bcl-2 mRNA表达水平下调,E-cadherin mRNA表达水平上调,ICE mRNA表达水平无明显变化,COX-2 mRNA未检测到。结论:塞来昔布能有效诱导前列腺癌DU-145细胞凋亡并使其侵袭力降低。
Objective: To study the effect of celecoxib on the apoptosis and invasiveness of prostate cancer DU-145 cells and to explore its possible mechanism. Methods: The morphology of apoptotic cells was detected by Hoechst 33342 / PI staining. The apoptosis of cells was detected by flow cytometry with Annexin V-FITC / PI double staining. The effect of celecoxib was detected by RT-PCR. Bcl-2, E-cadherin, ICE and COX-2 mRNA expression changes. Results: After Hoechst 33342 / PI double staining, the cells showed obvious apoptosis phenomenon. Flow cytometry confirmed that celecoxib could effectively induce apoptosis. The apoptotic rates induced by celecoxib at 0, 25, 50, 100 and 200 μmol / L were (1.10 ± 0.15)% and (3.87 ± 0.79) %, (10.59 ± 1.58)%, (22.50 ± 3.30)% and (33.85 ± 2.71)%, respectively. The apoptosis rate showed a concentration-dependent manner. The expression of E-cadherin mRNA was down- The level of ICE mRNA expression did not change significantly, COX-2 mRNA was not detected. Conclusion: Celecoxib can effectively induce the apoptosis of prostate cancer DU-145 cells and decrease its invasiveness.