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鼻粘膜被认为是呼吸道阻止抗原侵袭的第一道防线。为了更好地了解免疫活性细胞在上呼吸道防御和抵抗微生物侵袭中的作用,本研究调查了三种不同状态,即无病原菌(GF)、无特异性病原体(SPF)和一般状态(CV)豚鼠鼻粘膜中免疫活性细胞的分布状况。用抗IgG、IgA、IgM多克隆抗体和Lyt-1、Lyt-2、Mac-1单克隆抗体,通过间接过氧化物酶免疫染色法和普鲁氏兰染色法检测免疫活性细胞和肥大细胞。发现CV鼠的鼻粘膜中存在Mac-1~+细胞、肥大细胞和所有类型的淋巴细胞亚群。SPF鼠的鼻粘膜中也存在各种类型的细胞,但在数量上均少于CV鼠。GF鼠的鼻粘膜有少量
Nasal mucosa is considered the first line of defense against the invasion of the respiratory tract by antigens. In order to better understand the role of immunocompetent cells in the defense of the upper respiratory tract and in the resistance to microbial invasion, the present study investigated three different states, namely, pathogen free (GF), nonspecific pathogen (SPF) and general state (CV) guinea pigs Distribution of immunocompetent cells in nasal mucosa. Immunocompetent cells and mast cells were detected by indirect peroxidase immunostaining and Prussian blue staining with anti-IgG, IgA, IgM polyclonal antibodies and Lyt-1, Lyt-2, Mac-1 monoclonal antibodies. Mac-1 ~ + cells, mast cells and all types of lymphocyte subsets were found in the nasal mucosa of CV rats. There are also various types of cells in the nasal mucosa of SPF mice, but less in number than in CV rats. There is a small amount of nasal mucosa in GF rats