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随着脉冲电泳技术的发展,越来越多的链霉菌线性质粒被发现.链霉菌线性质粒的大小范围是9～640kb,末端为共价结合蛋白质并存在反向重复结构.其复制包括了中部起始的双向复制和末端蛋白起始的补了复制,这与同为末端共价结合蛋白质结构的腺病毒有显著不同.线性质粒可能有多种起源,它们既能以线状又能以环状形式复制,在接合转移及染色体整合过程中占有遗传优势,对链霉菌线性质粒的深入研究将有助于开发出一类新的原核生物线性载体系统.
With the development of pulse electrophoresis technology, more and more streptomyces linear plasmids have been found.The streptomyces linear plasmids range in size from 9 to 640kb, with covalently bound proteins at the ends and an inverted repeat structure.The replication includes the middle part The initial bidirectional replication complements the priming of the terminal protein, which is significantly different from adenoviruses that share the same structure as the covalently bound protein.The linear plasmids may have multiple origins, both linear and circular Form replication, occupying a genetic advantage in the process of junctional transfer and chromosomal integration. In-depth study on the streptomyces linear plasmids will help to develop a new class of prokaryotic linear vector systems.