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目的：观察釉原蛋白（Ａｍ）基因在大鼠牙胚组织发育过程中的表达。方法：采用原位杂交的方法，检测大鼠孕期１７、１８、１９ｄ和出生后１、３、５、７、９ｄ牙胚中成釉细胞ＡｍｍＲＮＡ的表达。结果：从出生后１ｄ至出生后９ｄ大鼠第一磨牙牙胚中均检测到ＡｍｍＲＮＡ的表达，其中出生后第５ｄ表达最高，成釉细胞分泌完成后Ａｍ的表达下降。成牙本质细胞在发育的任何时期均未见到Ａｍ的表达。结论：釉原蛋白基因的转录只发生在牙胚发育的早期，前成釉细胞极化阶段和成釉细胞分泌阶段；发育成熟的釉基质中没有釉原蛋白。成牙本质细胞不表达釉原蛋白基因。
Objective: To observe the expression of amyloid protein (Am) gene in the development of rat tooth germ tissue. Methods: In situ hybridization was used to detect the expression of AmmRNA in ameloblasts of rat embryos at 17,18,19 d and 1,3,5,7 and 9 d after birth. Results: The expression of AmmRNA was detected in the first molar tooth germ from 1 day after birth to 9 days after birth. The expression of Ammon protein was highest on the 5th day after birth and the expression of Am was decreased after the ameloblastment. The odontoblasts showed no expression of Am at any stage of development. CONCLUSION: The transcription of amelogenin gene occurs only in the early stages of tooth germ development, pre-ameloblastogenesis and ameloblast sequestration stage; there is no amelogenin in the mature enamel matrix. Odontoblasts do not express amelogenin genes.