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为研究电离辐射与人肿瘤细胞B7.1分子表达之间的关系 ,探讨肿瘤细胞在照射后免疫原性增强的机理。采用间接免疫荧光 流式细胞仪测定技术 ,用 30、4 0、5 0、6 0Gyγ射线照射 5种肿瘤细胞和一种非肿瘤细胞后 ,观察不同培养时间 (2 4、4 8、72、96h)内这些细胞B7.1分子的表达水平。用3H TdR释放试验测定肿瘤细胞和淋巴细胞共反应后细胞毒活性。结果表明 ,未经照射的肿瘤细胞不表达B7.1分子。经 4 0Gy照射后 ,SMMC 772 1肝癌细胞、PC 12嗜铬神经瘤细胞、A 5 49肺癌细胞表达B7.1共刺激分子 ,与对照组相比有非常显著性差异 ,但SHG胶质瘤细胞和HOS骨肉瘤细胞经 6 0Gy照射后仍未B7.1分子的表达。淋巴细胞与表达B7.1分子的肿瘤细胞共反应后细毒胞活性明显高于未照射组 p <0 .0 1)。实际提示 ,γ射线能诱导人肿瘤细胞表达B7.1分子 ,增强肿瘤细胞的免疫原性
To study the relationship between ionizing radiation and the expression of B7.1 in human tumor cells, the mechanism of enhanced immunogenicity of tumor cells after irradiation was explored. Indirect immunofluorescence flow cytometry was used to detect the effect of different culture time (2 4, 4, 8, 72, 96 h) on 5 kinds of tumor cells and one kind of non-tumor cells irradiated with 30, 40, 50, ) Within these cells B7.1 molecule expression levels. The 3H TdR release assay was used to determine the cytotoxic activity of tumor cells and lymphocytes after co-reaction. The results show that non-irradiated tumor cells do not express B7.1 molecules. After 40 Gy irradiation, B7.1 costimulatory molecules were expressed in SMMC 772 1 hepatoma cells, PC 12 chromaffin neuroma cells and A 5 49 lung cancer cells, which were significantly different from the control group, but SHG glioma cells And HOS osteosarcoma cells after 60 Gy did not express B7.1 molecules. The cytotoxic activity of lymphocytes after co-reaction with B7.1-expressing tumor cells was significantly higher than that of non-irradiated cells (p <0.01). The actual tips, γ-ray can induce human tumor cells to express B7.1 molecules, enhance the immunogenicity of tumor cells