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表达马铃薯Y病毒(PVY)和马铃薯卷叶病毒(PLRV)双价外壳蛋白基因的马铃薯(Solanum tubero-sum L.)栽培品种“Favorita”和“虎头”,经摩擦接种PVY和用桃蚜接种PLRV后,观察症状并用ELISA测定病毒滴度。结果表明,两个品种转双价CP基因的各株系,接种病毒后表现无症状或症状轻微,其中PVY和PLRV平均滴度均较不转基因对照植株低。不同品种对PVY和PLRV的抗性比较表明,转双价CP基因的“Favorita”对PVY抗性较明显,而转双价CP基因的“虎头”则对PLRV抗性较对PVY抗性明显。不同转基因株系抗病毒水平不同。“Favorita”9个转双价CP基因株系中有6个株系PVY滴度较未转基因对照降低52.5％～90.0％,而“虎头”7个转双价CP基因株系中有4个株系PLRV含量较对照降低53.0％～98.0％。在抗性株系中还出现一些抗1种病毒或抗2种病毒的抗性较强的单株。
Favorita and tiger heads of potato (Solanum tubero-sum L.) cultivars that express the bivalent coat protein genes of potato y virus (PVY) and potato leafroll virus (PLRV) were inoculated with PVY by friction and inoculated with peach aphids After PLRV, symptoms were observed and virus titers were determined by ELISA. The results showed that the two strains of transgenic CP gene of each strain showed no symptom or slight symptom after inoculation with virus, and the average titer of PVY and PLRV was lower than that of non-transgenic control plants. Comparison of the resistance of different cultivars to PVY and PLRV showed that the resistance of PVY to PVY was more obvious than that of PVY, while the resistance of PVT to PLRV was more obvious than that of PVY . Different transgenic lines have different antiviral levels. The titer of PVY in 6 strains of “Favorita” 9 transgenic bivalent CP strains was 52.5% -90.0% lower than that of the non-transgenic control, while 4 of the 7 bivalent CP gene strains of “tiitou” The PLRV content of the strain was 53.0% ~ 98.0% lower than that of the control. In the resistant lines, some plants resistant to 1 virus or 2 viruses were also found.