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用携带植物抗病毒基因表达载体ｐＢＴＵ的农杆菌转化油菜花培双低品种Ｈ０９０．Ｈ１６６，获得了大量转基因植株。载体质粒ｐＢＴＵ上有卡那霉素抗性标记基因（ＮＰＴⅡ）和ＣａＭ３５Ｓ启动子控制的芜菁花叶病毒ＴｕＭＶ－ｃｐ外壳蛋白基因，用品种Ｈ０９０和Ｈ１６６的带柄子叶与携带载体质粒的农杆菌共培养后，将其转移到含有６－ＢＡ１－１０ｍｇ／ｌ的ＭＳ培养基中，７～１５天后又将其转移到含有６－ＢＡ１－１０ｍｇ／ｌ的ＭＳ加卡那霉素１０ｍｇ的培养基上培养筛选２～３周后，在子叶柄基部出现小丛芽，将其切下转入Ｂ５加卡那霉素１０ｍｇ的培养基中进一步筛选并使转化体生根，将生根的正常绿色植株移栽于花盆中，接种ＴｕＭＶ进行攻毒试验，这些植株未出现发病现象，将未发病的自交种子，播种于含有卡那霉素的Ｂ５培养基上再一次进行抗性鉴定，均未出现白化苗。结果表明：卡那霉素抗性标记基因（ＮＰＴⅡ）已转入油菜植株。
Agrobacterium tumefaciens strain H090 was transformed with Agrobacterium tumefaciens harboring plant anti - virus gene expression vector pBTU. H166, obtained a large number of transgenic plants. The vector plasmid pBTU has the kanamycin resistance marker gene (NPTII) and the CaM35S promoter-controlled TuMV-cp coat protein gene of turnip mosaic virus, with the cotyledons of cultivars H090 and H166 and the vector carrying Agrobacterium After co-cultivation, they were transferred to MS medium containing 6-BA 1 to 10 mg / l, which was in turn transferred to medium containing 10 mg of MS-gabcakamycin 6-10 mg / l after 7 to 15 days After 2 to 3 weeks of culture selection, small clusters of shoots emerged at the base of the cotyledonary petiole and were excised into medium supplemented with 10 mg of B5 plus kanamycin for further screening and transformants were rooted, rooted normal green plants were removed The plants were planted in flower pots and inoculated with TuMV for challenge test. No occurrence of these plants occurred. The uninfected self-seeded seeds were sown again on kanamycin-containing B5 medium and no resistance was detected Albino seedlings. The results showed that the kanamycin resistance marker gene (NPTⅡ) was transferred into rape plants.