把拮抗性放綫菌接种在黄豆餅粉-葡萄糖液体培养基中,在25℃振蕩培养(160轉/分)72小时,用离心机除去菌絲,取清液作紙上層析的样品。先用杯碟法測定發酵液的抑菌力,用枯草菌作測定菌,抑菌圜在20—30毫米时,适于作層析样品;抑菌圜小于20毫米时效价太低,得不到圖譜;抑菌圜 Antagonistic actinomycetes were inoculated in soybean cake powder-glucose liquid medium, and cultured at 160 ° C for 72 hours with shaking (160 rpm). The mycelium was removed by a centrifuge and the supernatant was taken as a sample for paper chromatography. Bacillus subtilis was used to determine the bacteriostasis of the fermentation broth by the cup and saucer method. When the bacteriostasis was between 20 and 30 mm, the bacteriostasis was suitable for the chromatographic sample. When the bacteriostatic effect was less than 20 mm, the titer was too low, To map; antibacterial 圜