为防止组织切片从载玻片上脱落,目前一般使用铬矾明胶帖片.最近,我们发现免疫组织化学反应后的组织切片,用铬矾明胶帖片,经常规脱水、透明、封片后,其阳性反应产物随着时间的推移而发生褪色,影响到切片的保留和结果的观察.为此,本文用大鼠下丘脑的组织切片,在进行抗摧产素的免疫组织化学反应和硫酸镍铵加强的二氨基联苯胺(DAB)呈色,分别用单纯明胶或含不同浓度的铬矾明胶贴片,观察阳性产物消褪情况. In order to prevent tissue sections from shedding slides, the current general use of chrome alum gelatin posts. Recently, we found that immunohistochemical tissue sections, with chromium alum gelatin posts, after conventional dehydration, transparent, sealing, the Positive reaction products fade over time, affecting the retention of the section and the observation of the results.To this end, we use the rat hypothalamic tissue sections in the anti-erythropoietin immunohistochemical reaction and ammonium sulfate Fortified diaminobenzidine (DAB) staining, respectively, with a simple gelatin or containing different concentrations of chromium alum gelatin patch, observe the fading of positive products.