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目的调查2014年厦门地区市售牡蛎中GⅠ型、GⅡ型诺如病毒的污染状况,为食用诺如病毒污染的牡蛎引起的胃肠炎提供预警信息和防控建议。方法 2014年1月-2014年11月对厦门地区市售牡蛎进行连续采样,利用实时荧光定量RT-PCR法对样品中诺如病毒进行检测,确定牡蛎中诺如病毒污染状况及基因型分布状况。结果 198份牡蛎样品中诺如病毒总检出率为22.7%,GⅠ型No Vs检出率为6.1%,GⅡ型No Vs检出率为9.1%,GⅠ型和GⅡ型No Vs同时检出率为7.1%;消化腺中诺如病毒RNA载量水平为400 copy/g~485 000 copy/g,均值为9 500 copy/g。结论 2014年厦门地区市售牡蛎的诺如病毒污染情况较严重,GⅠ型和GⅡ型No Vs均有污染,建议相关部门采取措施,降低人群感染诺如病毒的风险。
Objective To investigate the status of GⅠ and GⅡ Norovirus in commercial oysters in Xiamen in 2014 and provide early warning information and prevention and control advice for gastroenteritis caused by Norovirus infected oyster. Methods From January 2014 to November 2014, continuous sampling of commercially available oysters in Xiamen was carried out. Norovirus was detected in samples by real-time fluorescence quantitative RT-PCR to determine the status of norovirus infection in oysters and the distribution of genotypes . Results The total detection rate of norovirus in 198 oyster samples was 22.7%, the detection rate of GV Ⅰ No Vs was 6.1%, the detection rate of G Ⅱ No Vs was 9.1%, the detection rates of GV Ⅰ and GⅡ No Vs Was 7.1%. The content of norovirus RNA in digestive gland was 400 copy / g ~ 485 000 copy / g with an average value of 9 500 copy / g. Conclusions In 2014, Xiamen City oysters were more likely to have norovirus infection, and both GⅠ and G Ⅱ No Vs were contaminated. Relevant departments are suggested to take measures to reduce the risk of norovirus infection in the population.