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制备了聚谷氨酸修饰玻碳电极,通过循环伏安法和差分脉冲伏安法研究了槲皮素在该修饰电极上的电化学行为。在pH 5.00的B-R缓冲液中,槲皮素在修饰电极上于0.28 V(vs Ag/AgCl)电位处产生一个灵敏的DPV阳极氧化峰,氧化峰电流与槲皮素的浓度在1.0×10-8～5×10-5 mol/L的范围内呈良好的线性关系,最低检测限为4.0×10-9 mol/L。实验表明,聚谷氨酸修饰电极可提高槲皮素的检测灵敏度,该电极用于芦丁水解产物中槲皮素的检测,回收率为103.4%～104.5%。
Polyglutamic acid modified glassy carbon electrode was prepared. The electrochemical behavior of quercetin at the modified electrode was studied by cyclic voltammetry and differential pulse voltammetry. In pH5.00 BR buffer, quercetin produced a sensitive DPV anodic oxidation peak at 0.28 V (vs Ag / AgCl) potential at the modified electrode. The peak current of oxidation and the concentration of quercetin were between 1.0 × 10- The linear range was from 8 to 5 × 10-5 mol / L with the detection limit of 4.0 × 10-9 mol / L. Experiments show that polyglutamic acid modified electrode can improve the detection sensitivity of quercetin, the electrode rutin hydrolyzate quercetin detection, the recovery was 103.4% to 104.5%.