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Objective:To determine whether alpha lipoic acid(LA)can effectively protect lenses from hydrogen peroxide(H_2O_2)-induced cataract.Methods:Lens from adult Sprague-Dawley rats were cultured in 24-well plates and treated without or with 0.2 mM of H_2O_2,0.2 mM of H_2O_2 plus 0.5 mM.1.0 mM.or 2.0 mM of LA for 24 h.Cataract was assessed using cross line grey scale measurement.Superoxide dismutase(SOD).glutathione(GSH-Px).lactate dehydrogenase(LDH). and maloudialdehyde(MDA)activity or level in lens homogenates was measured.Apoptosis of lens epithelial cells in each group were detected by Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling(TUNEL) Assay.Results:A total of 0.2 mM of H_2O_2 induced obvious cataract formation and apoptosis in lens’ epithelial cells,but 0.5-2.0 mM of LA could block the effect of 0.2 mM H_2O_2 in inducing cataract and apoptosis.Furthermore.0.2 mM ol H_2O_2 significantly decreased SOD.GSH-Px,and LDH activity and significant increased MDA level in the lens,but 0.5-2.0 mM of LA blocked the effect of 0.2 mM H_2O_2.One mM of LA was found to be the most effective. Conclusions:LA can protect lens from H_2O_2-induced cataract.LA exerts protective effects through inhibition of lens’ epithelial cell apoptosis and activation of anti-oxidative enzymes.
Objective: To determine whether alpha lipoic acid (LA) can effectively protect lenses from hydrogen peroxide (H 2 O 2) -induced cataract. Methods: Lens from adult Sprague-Dawley rats were cultured in 24-well plates and treated without or with 0.2 mM of H 2 O 2 , 0.2 mM of H202 plus 0.5 mM 0.1 mM. 2.0 mM of LA for 24 h. Cataract was assessed using cross line gray scale measurement. Superoxide dismutase (SOD). Glutathione (GSH-Px). Lactate dehydrogenase (LDH). and maloudialdehyde (MDA) activity or level in lens homogenates was measured. Apoptosis of lens epithelial cells in each group were detected by Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Assay. Results: A total of 0.2 mM of H 2 O 2 induced significant cataract formation and apoptosis in lens’ epithelial cells, but 0.5-2.0 mM LA could block the effect of 0.2 mM H 2 O 2 in inducing cataract and apoptosis.Furthermore.0.2 mM ol H 2 O 2 significantly decreased SOD.GSH-Px, and LDH activity and significantly increased MDA level in the lens, but 0.5-2.0 mM of LA blocked the effect of 0.2 mM H 2 O 2. One mM of LA was found to be the most effective. Conclusions: LA can protect lens from H 2 O 2 -induced cataract. LA exerts protective effects through inhibition of lens’ epithelial cell apoptosis and activation of anti-oxidative enzymes.