目的　探讨nm2 3 H1基因突变与人类肺癌发生和转移的关系。方法　常规提取肺癌基因组DNA作为模板 ,PCR扩增nm2 3 H1基因 ,变性PCR产物 ,6%非变性聚丙烯酰胺凝胶SSCP电泳 ,银染后分析结果。结果　成功地从 11例肺癌肿瘤组织及相应正常组织基因组中扩增出nm2 3 H1基因 ,并发现 1例 (转移淋巴结 )存在 2 3nm H1基因突变。结论　nm2 3 H1基因是转移抑制基因 ,此基因的突变与肺癌转移相关。 Objective To investigate the relationship between the mutation of nm23H1 gene and the occurrence and metastasis of human lung cancer. Methods Routinely extracted lung cancer genomic DNA as template, PCR amplification of nm2 3 H1 gene, denatured PCR products, 6% non-denaturing polyacrylamide gel SSCP electrophoresis, silver staining results. Results We successfully amplified the nm23H1 gene from 11 lung cancer tumor tissues and the corresponding normal tissue genomes, and found that there was a 23nm H1 gene mutation in 1 case (metastatic lymph node). Conclusion The nm23H1 gene is a metastasis suppressor gene. Mutation of this gene is associated with metastasis of lung cancer.