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目的探讨精囊蛋白1(Sg1)在膀胱癌和癌旁组织中的表达及临床意义。方法收集病理诊断的20对膀胱癌和癌旁组织,应用Western blot和实时定量PCR技术检测Sg1的表达。同时,收集病理诊断的46例膀胱癌和15例癌旁组织的蜡块标本,应用免疫组化方法检测Sg1蛋白的表达,并进一步研究了Sg1与分级、分期以及转移的相关性。结果膀胱癌组织中Sg1mRNA的表达低于癌旁组织(P<0.05);Sg1蛋白表达亦表现出相同的趋势。膀胱癌组织中Sg1的表达降低与肿瘤细胞的分期、分级和转移等具有明显的关系(P<0.05或P<0.01)。结论 Sg1蛋白在膀胱癌中的异常表达与其发生、发展密切相关。
Objective To investigate the expression and clinical significance of seminal vesicle protein 1 (Sg1) in bladder cancer and adjacent normal tissues. Methods Twenty bladder cancer tissues and paracancerous tissues were collected. The expression of Sg1 was detected by Western blot and real-time quantitative PCR. At the same time, the specimens of pathological diagnosis of 46 cases of bladder cancer and 15 cases of paracancerous tissues were collected. The expression of Sg1 protein was detected by immunohistochemistry. The correlation between Sg1 and grading, staging and metastasis was further studied. Results The expression of Sg1 mRNA in bladder cancer tissues was lower than that in paracancerous tissues (P <0.05). The expression of Sg1 protein also showed the same trend. The decreased expression of Sg1 in bladder cancer has a significant relationship with the staging, grading and metastasis of tumor cells (P <0.05 or P <0.01). Conclusion The abnormal expression of Sg1 in bladder cancer is closely related to its occurrence and development.