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从‘嘎拉’苹果中克隆了一个NAC转录因子基因MdNAC143(序列号:MDP0000334047),其开放阅读框为924 bp,编码308个氨基酸,预测蛋白质的分子量为35.59 k D,等电点为6.32。结构域分析表明,MdNAC143蛋白N端含有保守的NAC结构域。酵母双杂交结果表明MdNAC143的全长及C端具有转录激活活性。荧光定量PCR分析表明,MdNAC143在苹果的各个组织均有表达,其中在叶片和花中表达相对较高;MdNAC143的表达明显受盐胁迫的诱导。将MdNAC143遗传转化‘王林’苹果愈伤组织,进行抗盐表型鉴定后发现,MdNAC143在愈伤组织中过量表达后能明显提高对盐胁迫的抗性。
A NAC transcription factor gene MdNAC143 (MDP0000334047) was cloned from Gala apple and its open reading frame was 924 bp, encoding a protein of 308 amino acids. The molecular weight of the predicted protein was 35.59 kD and the isoelectric point was 6.32. Domain analysis showed that the N-terminal of MdNAC143 protein contains a conserved NAC domain. Yeast two-hybrid results showed MdNAC143 full-length and C-terminal transcriptional activation. Quantitative real-time PCR analysis showed that MdNAC143 was expressed in all tissues of apple, and its expression in leaves and flowers was relatively high. The expression of MdNAC143 was significantly induced by salt stress. MdNAC143 was genetically transformed into ’Wanglin’ apple callus and identified by salt phenotype. The results showed that MdNAC143 could significantly increase the resistance to salt stress after overexpression in callus.