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进行了四唑(鎓)(tetrazolium)染色法快速测定巴西橡胶种子生活力的试验。预备试验是确定种子提前泡水的有效时间,以及研究染色适宜的浓度、时间和温度。种子预先泡水4小时的,染色浅而不规则;泡水16小时的,染色较好。种子浸泡在1％浓度的2,3,5,——三苯基四唑(鎓)化氯(2,3,5——triphenyel tetrazolium chloride)溶液中,温度40℃,历时2小时的,胚和胚乳染色最好,染成一致的红色。对不同质量种子应用这种四唑染色技术进行测试所得到的结果,与发芽试验的结果相差显著。用此法在24小时内可以得到结果,而正常的发芽试验至少需要3个星期。
The tetrazolium (tetrazolium) staining method was used to test the viability of Brazil rubber seeds. The preliminary test is to determine the effective time of seed soaking the water ahead of time, and to study the appropriate concentration, time and temperature for staining. The seeds were soaked in water for 4 hours in advance, and the dyeing was light and irregular; the soaking for 16 hours was better. The seeds were soaked in a 1% strength solution of 2,3,5-triphenyldi tetrazolium chloride at a temperature of 40 ° C for 2 hours, And endosperm staining the best, dyed red. The results of applying this tetrazolium staining technique to different quality seeds differed significantly from the germination test. This method gives results within 24 hours, while normal germination tests require at least 3 weeks.