论文部分内容阅读
目的:通过制备As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒来研究热化疗对乳腺癌的治疗效果。方法:采用改良的化学沉淀-浸渍法制备As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,通过透射电镜、扫描电镜、能谱仪、原子荧光光谱仪来表征;检测As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒的释药水平及在交变磁场中的升温能力;通过MTT比色法和流式细胞仪检测化疗组、热疗组及热化疗组乳腺癌的治疗效果。结果:制备的复合纳米粒粒径为20~40 nm;复合纳米粒体外升温能达到肿瘤的有效治疗温度(41~46℃);释药缓慢,48 h释药为14.67%。结论:采用改良的化学沉淀-浸渍法可以成功制备As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,体外实验证明该复合纳米粒联合交变磁场热疗对乳腺癌细胞具有很强的生长抑制和凋亡诱导作用。
OBJECTIVE: To study the therapeutic effect of thermochemotherapy on breast cancer by preparing As2O3 / Mn0.5Zn0.5Fe2O4 composite nanoparticles. Methods: As2O3 / Mn0.5Zn0.5Fe2O4 composite nanoparticles were prepared by modified chemical precipitation-impregnation method and characterized by transmission electron microscopy, scanning electron microscopy, energy dispersive spectroscopy and atomic fluorescence spectrometry. As2O3 / Mn0.5Zn0.5Fe2O4 composite nanoparticles Release level and the ability to heat up in alternating magnetic field. MTT colorimetric assay and flow cytometry were used to detect the therapeutic effect of breast cancer in chemotherapy group, hyperthermia group and thermochemotherapy group. Results: The particle size of the prepared composite nanoparticles was 20-40 nm. The effective temperature of the composite nanoparticles could reach the effective therapeutic temperature (41-46 ℃) when the nanoparticles were heated in vitro. The drug release was slow and the release rate was 14.67% at 48 h. CONCLUSION: As2O3 / Mn0.5Zn0.5Fe2O4 composite nanoparticles can be prepared successfully by modified chemical precipitation-impregnation method. In vitro experiments show that the composite nanoparticles with alternating magnetic hyperthermia have strong growth inhibition and apoptosis on breast cancer cells Induction.