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本文对经KMnO_4、H_2O_2刺激掘氏疫霉(Phytophthora drechsleri)卵孢子萌发建立的单卵孢株群体应用于掘氏疫霉遗传研究的可行性做了研究。2个掘氏疫霉菌株(A_1、A_2交配型各1株)经聚碳膜间隔配对产生的卵孢子用KMnO_4、H_2O_2以及不用上述氧化剂处理分别建立K、H、C 3个单卵孢株群体(卵孢子萌发率8—16%)。在群体水平上对来自同一亲本的上述3个群体生物学性状比较结果表明,H_2O_2处理刺激卵孢子萌发不影响生长速度、菌落形态、耐35℃高温生长、致病力及交配型的遗传稳定性,所建立的单卵孢株群体可用于上述性状的遗传研究;KMnO_4处理导致掘氏疫霉A_1和A_2交配型在自交后代各自分离为A_1、A_2、A_1A_2和A_2、A_1A_2,但不影响其他生物学性状的遗传稳定性,所建立的单卵孢株群体可用于除交配型性状以外的其他生物学性状的遗传研究,不宜用于交配型正常遗传规律的研究。用KMnO_4处理掘氏疫霉A_1或A_2交配型菌株单株自交产生的卵孢子,从A_1菌株自交后代获得A_2和A_1A_2以及从A_2菌株自交后代获得A_1A_2交配型单卵孢株的结果说明,掘氏疫霉异宗配合菌株体内可能同时包含控制A_1和A_2交配型的遗传因子,在正常条件下有1种交配型遗传因子不表达。KMnO_4处理可望作为揭示交配型遗传本质的有用试验手段。
In this paper, we investigated the feasibility of applying the mono-oospores population established by KMnO_4 and H_2O_2 to spawn Phytophthora drechsleri to the research of Phytophthora sojae. Two populations of Phytophthora dysenteriae strains (1 mating type of A_1 and A_2), respectively, produced oocyte sporozoites by KMnO 4, H 2 O 2 and no oxidant treatment. (Oospore germination rate of 8-16%). The comparison of the biological traits of the three groups from the same parent at the population level showed that H 2 O 2 treatment stimulated the spore germination without affecting the growth rate, colony morphology, growth at 35 ℃ resistance, pathogenicity and the genetic stability of mating type , And the established mono-oospore population could be used for genetic research on the above traits. KMnO_4 treatment led to the isolation of the mating type of Phytophthora datum A_1 and A_2 into A_1, A_2, A_1A_2, A_2 and A_1A_2, respectively, but did not affect other Genetic stability of biological traits, the established single-oospore population can be used for other than mating sex traits of other biological properties of genetic research should not be used for mating normal genetic law. The result of inoculation with KMnO_4 on the inbred oospores generated from the mating strains of Phytophthora dysenteriae A_1 or A_2 and obtaining A_2 and A_1A_2 from the selfing offspring of A_1 strain and the mating of the A_1A_2 mating type from the progeny of the A_2 strain , Phytophthora dysenteriae heterotrophic strains may contain both genetic factors that control the mating type of A_1 and A_2 in vivo, and one mating type genetic factor is not expressed under normal conditions. KMnO_4 treatment is expected to serve as a useful experimental means to reveal the genetic nature of mating type.