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Objective:To evaluate of hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells(MCF-7/Dox)in cytotoxicity apoptosis and P-glycoprotein(Pgp)expression in combination with doxorubicin.Methods:The cytotoxic properties.50%inhibition concentration(IC_(50))and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin(MCF-7/Dox)cells were determined using MTT assay.Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange.Immunocytochemistry assay was performed to determine the level and localization of Pgp.Results:Single treatment of hesperidin showed cytotoxic activity on MCF-7/Dox cells with IC_(50)value of 11μmol/L.Thus,combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect(CI>1.0).Hesperidin did not increase the apoptotic induction,but decreased the Pgp expressions level when combined with doxorubicin in low concentration.Conclusions:Hesperidin has cytotoxic effect on MCF-7/Dox cells with IC_(50)of 11μmol/L.Hesperidin did not increased the apoptotic induction combined with doxorubicin.Cochemotherapy application of doxorubicin and hesperidin on MCF-7/Dox cells showed synergism effect through inhibition of Pgp expression.
Objective: To evaluate the hesperidin to overcome resistance of doxorubicin in MCF-7 resistant doxorubicin cells (MCF-7 / Dox) in cytotoxicity apoptosis and P-glycoprotein (Pgp) expression in combination with doxorubicin. Methods: The cytotoxic properties. 50% inhibition (IC 50) and its combination with doxorubicin in MCF-7 cell lines resistant to doxorubicin (MCF-7 / Dox) cells were determined using a MTT assay. Apoptosis induction was examined by double staining assay using ethidium bromide-acridine orange. Immunocytochemistry assay was performed to determine the level and localization of Pgp. Results: Single treatment of hesperidin showed cytotoxic activity on MCF-7 / Dox cells with IC 50 (50) value of 11 μmol / L. Thus, combination treatment from hesperidin and doxorubicin showed addictive and antagonist effect (CI> 1.0). Hesperidin did not increase the apoptotic induction, but decreased the Pgp expression level when combined with doxorubicin in low concentration. Conclusions: Hesperidin has cytotoxic ef fect on MCF-7 / Dox cells with IC 50 (50) of 11 μmol / L. Hesperidin did not increased the apoptotic induction combined with doxorubicin. Cochemotherapy application of doxorubicin and hesperidin on MCF-7 / Dox cells showed synergism effect through inhibition of Pgp expression .