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目的：探讨解偶联蛋白－１（ＵＣＰ－１）和β３－肾上腺素能受体（β３－ＡＲ）基因在肥胖人群中的变异及其对肥胖患者基础代谢率（ＢＭＲ）和体重指数（ＢＭＩ）的影响。方法：应用聚合酶链反应产物限制性酶切片段长度多态性检测法测定ＵＣＰ－１基因Ａ→Ｇ（－３８２６）变异和β３－ＡＲＴｒｐ６４Ａｒｇ突变并作基因分型，分析突变等位基因与ＢＭＲ和ＢＭＩ的相关性。结果：本研究肥胖人群ＵＣＰ－１野生型等位基因（Ａ）和突变型等位基因（Ｇ）的频率分别为０．７１７０和０．２８９９。β３－ＡＲ野生型等位基因（Ｔｒｐ６４）和突变型等位基因（Ａｒｇ６４）的频率分别为０．８４４２和０．１５５８。ＵＣＰ－１和β３－ＡＲ基因均有变异的肥胖患者与该两基因均无变异的肥胖患者相比，ＢＭＲ降低和ＢＭＩ增高分别显示极显著意义（Ｐ＜０．０１）和显著意义（Ｐ＜０．０５）。结论：ＵＣＰ－１基因Ａ→Ｇ（－３８２６）变异和β３－ＡＲＴｒｐ６４Ａｒｇ突变与肥胖患者ＢＭＲ降低和体重增加相关，两基因的变异对ＢＭＲ和ＢＭＩ的影响存在加合作用。
Objective: To investigate the variation of UCP-1 and β3-adrenergic receptor (β3-AR) gene in obese subjects and their correlations with the baseline metabolic rate (BMR) and body mass index (BMI) )Impact. Methods: The UCP-1 gene A → G (-3826) mutation and β3-ARTrp64Arg mutation were genotyped by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The relationship between the mutation allele and BMR And BMI relevance. Results: The frequencies of UCP-1 wild type allele (A) and mutant allele (G) in obese population in this study were 0.7170 and 0.2899, respectively. The frequencies of β3-AR wild-type allele (Trp64) and mutant allele (Arg64) were 0.8442 and 0.1558, respectively. Compared with obese patients who had no mutation in both UCP-1 and β3-AR genes, the decrease of BMR and the increase of BMI were significant (P <0.01) and significant (P < 0.05). CONCLUSION: Mutations of UCP-1 A → G (-3826) and β3-ARTrp64Arg are associated with decreased BMR and weight gain in obese patients. There is an additive effect on the BMR and BMI in the two gene variants.