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目的 :探讨血管瘤与内皮素 1 (ET - 1 )、一氧化氮 (NO)和细胞间粘附分子 1 (ICAM - 1 )等血管内皮细胞活性因子的相互关系。方法 :选择 6 0例血管瘤患者 ,手术切除瘤体组织和周围少量正常组织 ,行HE染色 ,分为血管瘤增殖期和退化期两实验组。采用放射免疫分析检测血管瘤体和正常组织内ET - 1 ,采用酶联免疫分析检测细胞间粘附分子 1 (ICAM - 1 ) ,采用Geriss法检测血浆NO水平。结果 :增殖期血管瘤较周围正常组织高水平表达ET - 1和ICAM - 1 ,低水平表达NO。退化期血管瘤瘤体ET - 1、ICAM - 1和NO水平同周围正常组织无明显改变。结论 :血管瘤的发生发展同血管内皮细胞活性因子密切相关 ,ET - 1、ICAM - 1和NO等血管活性因子的检测有助于血管瘤发病机制的探讨。
Objective: To investigate the relationship between hemangiomas and the activity of vascular endothelial cells (ET - 1), nitric oxide (NO) and intercellular adhesion molecule 1 (ICAM - 1). Methods: Totally 60 patients with hemangiomas were selected for surgical resection of tumor tissue and a small amount of normal tissue. HE staining was used to divide them into proliferative phase and degenerative phase of hemangiomas. Radioimmunoassay (RIA) was used to detect the expression of ET - 1 in vascular tumor and normal tissues. ICAM - 1 was detected by enzyme - linked immunosorbent assay (ELISA), and plasma NO level was measured by Geriss method. Results: The proliferating hemangiomas expressed ET - 1 and ICAM - 1 at high level and NO at low level compared with normal tissues. The levels of ET - 1, ICAM - 1 and NO in the degenerative hemangiomas were similar to those in the surrounding normal tissues. Conclusion: The occurrence and development of hemangiomas are closely related to the activity of vascular endothelial cells. The detection of vascular endothelial growth factor such as ET - 1, ICAM - 1 and NO contributes to the pathogenesis of hemangiomas.