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建立了一种分子印迹-化学发光阵列传感器测定甘氨酸的新方法.该方法以甘氨酸为模板分子,合成了分子印迹聚合物微球,将该聚合物微球固定在96孔板上,用它来识别丹磺酰氯标记的甘氨酸(Dns-Gly).最后加入化学发光试剂(TCPO-H2O2-咪唑),测量相对化学发光强度定量检测甘氨酸.在最佳试验条件下,相对化学发光强度和甘氨酸的浓度在0.2～60μmol/L范围内成良好的线性关系,相关系数为r=0.9972,方法的检出限为0.07μmol/L,对1μmol/L甘氨酸溶液进行11次平行测定,相对标准偏差为3.3%(n=11).由于以甘氨酸为模板分子合成出来的分子印迹聚合物空腔比较小,避免了非特异性吸附,使它在识别丹磺酰氯标记的甘氨酸时特异性、响应速度和灵敏度都有所增强.
A new method for the determination of glycine by molecular imprinting-chemiluminescence array sensor was established.The molecularly imprinted polymer microspheres were synthesized by using glycine as a template molecule and the polymer microspheres were immobilized on a 96- Dansyl chloride-labeled glycine (Dns-Gly) was identified. Finally, chemiluminescence reagent (TCPO-H2O2-imidazole) was added to measure the relative chemiluminescence intensity of glycine. Under the optimum conditions, the relative chemiluminescence intensity and glycine concentration In the range of 0.2 ~ 60μmol / L, the correlation coefficient was r = 0.9972. The detection limit of this method was 0.07μmol / L. The relative standard deviation of the 1μmol / L glycine solution was 11 times and the relative standard deviation was 3.3% (n = 11). Since the molecularly imprinted polymer synthesized with glycine as a template has a relatively small cavity, nonspecific adsorption is avoided and its specificity, response speed and sensitivity in identifying Dansyl chloride-labeled glycine Enhanced.