疟原虫Rab蛋白是Ras超家族的一员,可见于疟原虫蛋白分泌通道的特定环节,其活性受GTP调节。为探讨应用Rab蛋白作为疟原虫蛋白分泌通道特异性标记的可能性,作者应用PCR鉴定了Pfrab8种不同的Rab基因,并对Rab6蛋白进行了鉴定。 作者根据参与形成Ras相关蛋白的GTP结合位点的保守序列,人工合成两套寡核苷酸引物序列,以恶性疟原虫3D7株基因组DNA和cDNA为模板进行PCR。将PCR The Plasmodium Rab protein, a member of the Ras superfamily, is found in specific parts of the pathogen of the parasite’s protein secretion and its activity is regulated by GTP. In order to explore the possibility of using Rab protein as a specific marker of the secretion pathway of Plasmodium protein, the authors identified 8 different Rab genes of Pfrab by PCR and identified Rab6 protein. Based on the conserved sequences of the GTP binding sites involved in the formation of Ras-related proteins, two sets of oligonucleotide primers were synthesized and PCRs were performed using genomic DNA and cDNA of Plasmodium falciparum 3D7 as a template. PCR