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目的探讨维生素D(VD)水平对哮喘患儿T细胞水平及免疫球蛋白水平影响。方法选取2014年-2015年在绍兴市中心医院就诊及住院的2岁~6岁支气管哮喘患儿60例,随机分为实验研究组(哮喘1组)及实验对照组(哮喘2组),各30例。哮喘1组在常规治疗的基础上给予维生素D3补充3个月。并选取门诊体检健康儿童60例作为健康对照组。对哮喘患儿治疗前后及健康组儿童体检时用化学发光法测定VD水平,免疫比浊法测定Ig A、Ig M和Ig G,流式细胞仪测定CD4+、CD8+、CD4+/CD8+T淋巴细胞水平。结果哮喘患儿VD水平及CD4+水平、CD4+/CD8+及Ig A水平均低于对照组,差异有统计学意义(P<0.05)。哮喘1组及哮喘2组治疗后CD4+/CD8+、Ig A高于治疗前,治疗后哮喘1组VD水平、CD4+、CD4+/CD8+、Ig A均高于哮喘2组,差异均有统计学意义(P<0.05)。VD水平与CD4+/CD8+、Ig A均呈正相关(r值分别为3.86、3.93,P<0.05)。结论哮喘患儿普遍存在维生素D缺乏、T细胞水平及免疫球蛋白水平异常,通过补充维生素D可以改变患儿体内T细胞水平及免疫球蛋白水平。
Objective To investigate the effect of vitamin D (VD) on T cell and immunoglobulin levels in asthmatic children. Methods Sixty children aged 2 to 6 years with bronchial asthma admitted to Shaoxing Central Hospital from 2014 to 2015 were randomly divided into experimental group (asthma group 1) and experimental control group (asthma group 2) 30 cases. Asthma group 1 was given vitamin D3 for 3 months on the basis of routine treatment. 60 outpatients were selected as healthy control group. The level of VD in children with asthma before and after treatment and the healthy group were measured by chemiluminescence method, Ig A, Ig M and Ig G were determined by immunoturbidimetry, and the levels of CD4 +, CD8 + and CD4 + / CD8 + T lymphocytes were determined by flow cytometry Level. Results The levels of VD, CD4 +, CD4 + / CD8 + and IgA in children with asthma were significantly lower than those in the control group (P <0.05). The levels of CD4, CD8 + and IgA in asthmatic group 1 and asthmatic group 2 after treatment were higher than those before treatment, and the levels of VD, CD4 +, CD4 + / CD8 + and IgA in asthmatic group 1 were significantly higher than those in asthmatic group 2 P <0.05). The level of VD was positively correlated with CD4 + / CD8 + and IgA (r = 3.86, 3.93, P <0.05). Conclusion The prevalence of vitamin D deficiency, abnormal T cell level and immunoglobulin levels in children with asthma can change the level of T cells and immunoglobulin levels in children with vitamin D supplementation.