目的：研究抗肿瘤抗生素Ｃ１０２７ 对人结肠癌ＨＣＴ８ 细胞凋亡相关基因表达的影响。方法：利用ｃＤＮＡ 排列（ｃＤＮＡａｒｒａｙｓ）、Ｎｏｒｔｈｅｒｎ 杂交、ＲＴＰＣＲ 检测技术。结果：用１０ ｎｍｏｌ·Ｌ－ １ Ｃ１０２７ 处理ＨＣＴ８ 细胞８ ｈ 后，利用ｃＤＮＡ 排列检测，发现Ｃ１０２７ 可以促进ＴＲＩＰ，ＴＲＡＦ３ ，ＤＲ４ ，ＭＣＨ６ ，ＭＣＨ４，Ａｐｏ３ ，ＤＲ５，ＡＢＬＬ，ＳＴＡＴ１ 等基因的表达，抑制ｒｈｏＣ的表达。用Ｎｏｒｔｈｅｒｎ 杂交及ＲＴＰＣＲ 也得到了同样结果。结论：Ｃ１０２７ 可能通过调节ＴＮＦ 受体家族有关的凋亡信号传导通路，诱导细胞凋亡，发挥抗瘤活性 Objective: To study the antitumor antibiotic C1027 on human colon cancer HCT 8 apoptosis-related gene expression. Methods: Using cDNA array (cDNAarrays), Northern hybridization, RT-PCR detection technology. Results: After treatment with 10 nmol·L-1 C1027 for 8 h, the expression of TRIP3, TRAF3, DR4, MCH6, MCH4, Apo3, DR5, ABLL and STAT1 Expression, inhibition of rhoC expression. The same result was obtained with Northern hybridization and RT-PCR. Conclusion: C1027 may induce apoptosis and exert anti-tumor activity by regulating apoptosis signal transduction pathway related to TNF receptor family