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目的：探讨本地区丙型肝炎病毒（ Ｈ Ｃ Ｖ） 感染者的基因分型。方法：应用逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ） 进行 Ｈ Ｃ Ｖ Ｒ Ｎ Ａ检测，并对其阳性产物进行 Ｈ Ｃ Ｖ 非结构５ 区的限制性片段长度多态性分析。结果：安徽省丙型肝炎患者 Ｈ Ｃ Ｖ 基因型有五种，即Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅱ／ Ⅲ型，其构成比分别为０ ．５３ ％ 、７５ ．６６ ％ 、１３ ．２３ ％ 、０ ．５３ ％ 、６ ．３５ ％ 。丙肝患者不同病种与 Ｈ Ｃ Ｖ 基因型分布差异无显著性（ Ｐ＞ ０ ．０５） ，献血员与输血后丙型肝炎患者间分布差异无显著性（ Ｐ＞ ０ ．０５） ，血透与输血后丙型肝炎患者间 Ｈ Ｃ Ｖ 基因型分布差异有显著性（ Ｐ＜ ０ ．０１） 。结论：安徽省以 Ｈ Ｃ ＶⅡ型为优势株，应加强对血透患者及献血员进行 Ｈ Ｃ Ｖ 筛检。
Objective: To investigate the genotyping of hepatitis C virus (H C V) in this area. Methods: Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect H C VR N A, and the positive products were analyzed by restriction fragment length polymorphism (PCR-RFLP). Results: There were five H C V genotypes in patients with hepatitis C in Anhui Province, namely Ⅰ, Ⅱ, Ⅲ, Ⅳ, Ⅱ / Ⅲ, and their constituent ratios were 0. 53%, 75. 66%, 13. 23%, 0. 53%, 6. 35%. There were no significant differences in the distribution of H C V genotypes between different disease types and hepatitis C patients (P> 0.05). There was no significant difference between hepatitis C patients and blood donors (P> 0.05) The distribution of H C V genotypes among patients with hepatitis C after transfusion was significant (P <0.01). Conclusion: H C V Ⅱ is dominant strain in Anhui Province. H C V screening of hemodialysis patients and blood donors should be strengthened.