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目的探讨辅助性T细胞9(Th9)在肝囊型包虫病患者慢性感染中的作用。方法通过流式细胞术和实时荧光定量PCR法分别检测2010年8月至2012年12月新疆医科大学第一附属医院33例囊型包虫病患者(CE)及20例健康体检者外周血Th9细胞和特异性转录因子PU.1的表达。结果 1)CE组Th9细胞占CD4+T的比值(CD3+CD4+IL-9+/CD4+T cells)(0.91%±0.57%)高于健康对照组(0.61%±0.2%),差异具有统计学意义(P=0.042);2)CE组PU.1、IL-9 mRNA的表达水平明显高于健康对照组,差异均有统计学意义(P<0.05)。结论 CE患者体内Th9/IL-9表达上调,Th9细胞可能参与了细粒棘球蚴慢性感染的免疫应答和调控。
Objective To investigate the role of helper T cell 9 (Th9) in chronic infection of patients with hepatic cystic echinococcosis. Methods 33 cases of cystic echinococcosis (CE) in the First Affiliated Hospital of Xinjiang Medical University from August 2010 to December 2012 were detected by flow cytometry and real-time fluorescence quantitative PCR. Th9 Expression of cell and specific transcription factor PU.1. Results 1) The ratio of Th9 cells to CD4 + T in CE group (0.91% ± 0.57%) was significantly higher than that in healthy controls (0.61% ± 0.2%) (P = 0.042). 2) The expression of PU.1 and IL-9 mRNA in CE group was significantly higher than that in healthy control group (P <0.05). Conclusions Th9 / IL-9 is up-regulated in CE patients, and Th9 cells may be involved in the immune response and regulation of chronic infection of Echinococcus granulosus.