目的：对人重组骨形态发生蛋白２（ｒｈ Ｂ Ｍ Ｐ２）和 β转化生长因子（ Ｔ Ｇ Ｆβ）在诱导成骨的协同作用进行探讨． 方法：２１０ 只 Ｂ Ａ Ｌ Ｂ／ｃ 小鼠随机分为 ３ 组，每组 ７０ 只，试验侧均位于右后肢，设立 ｒｈ Ｂ Ｍ Ｐ２／牛松质骨载体、单纯牛松质骨载体为对照组． 分别于术后 １２ ｈ ～２１ ｄ 共 １１ 个时间点取材，观察其诱导成骨过程． 结果：ｒｈ Ｂ Ｍ Ｐ２／ Ｔ Ｇ Ｆβ／牛松质骨载体组在诱导间充质细胞增殖、分化，软骨细胞及新生骨形成方面均早于ｒｈ Ｂ Ｍ Ｐ２／牛松质骨载体组，成骨量优于 ｒｈ Ｂ Ｍ Ｐ２／牛松质骨载体组，其碱性磷酸酶水平高峰较对照组提前，其组织钙含量明显高于对照组（ Ｐ＜ ０．０５）． 而单纯牛松质骨载体组在 ２１ ｄ仅出现了少量增殖的间充质细胞． 结论：ｒｈ Ｂ Ｍ Ｐ２ 和 Ｔ Ｇ Ｆβ在诱导成骨调节中存在着协同作用． OBJECTIVE: To investigate the synergistic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and β-transforming growth factor (TGF-β) on osteogenic induction. METHODS: A total of 210 B A L B / c mice were randomly divided into 3 groups (n = 70 in each group). The experimental groups were all on the right hindlimb. The rhB M P2 / bovine cancellous bone vector For the control group. The specimens were harvested at 11 time points from 12 h to 21 d after operation to observe the induction of osteogenesis. Results: rh B M P2 / T G Fβ / bovine cancellous bone vector group was earlier than rh B M P2 / bovine pine in inducing mesenchymal cell proliferation, differentiation, chondrocytes and newborn bone formation Compared with rhBMP-2 / bovine cancellous bone vector group, the osteogenic rate of osteogenic vector group was higher than that of control group, and the calcium content in tissue was significantly higher than that in control group (P <0. 05). However, only a small amount of proliferating mesenchymal cells appeared on the 21th day in the simple bovine cancellous bone vector group. Conclusion: rh B M P2 and T G Fβ have synergistic effect in inducing osteogenesis.