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以大豆品种“晋豆8号”为材料,采用组织培养方法研究了Ca(NO_3)_2对盐胁迫下大豆离体胚再生植株有机物质积累、膜脂过氧化和保护酶系统的影响.结果表明,适宜浓度的Ca(NO_3)_2处理,可促进一定盐胁迫下大豆再生植株内游离脯氨酸和可溶性糖的积累,以缓解盐胁迫造成的渗透伤害,并可促进保护酶系统超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,减少膜质过氧化产物丙二醛(MDA)含量,降低相对膜造性,减轻盐对膜的伤害.低盐胁迫时以Ca(NO_3)_2 15mM、高盐胁迫时以 Ca(NO_3)_2 10mM效果最佳.
The effects of Ca (NO_3) _2 on the accumulation of organic matter, membrane lipid peroxidation and protective enzyme system of soybean embryogenic regenerated plants under salt stress were studied using the soybean cultivar “Jin Dou 8” as material. The results showed that , Ca (NO_3) _2 at the proper concentration could promote the accumulation of free proline and soluble sugar in the regenerated soybean plants under certain salt stress, so as to relieve the osmotic damage caused by salt stress and promote the superoxide dismutase (SOD) and catalase (CAT) activities, and decreased the malondialdehyde (MDA) content of membrane peroxidation products, decreased the relative membrane formation and decreased the salt damage to the membrane. _2 15mM, the best effect of Ca (NO_3) _2 10mM under high salt stress.