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本文旨在探讨急性高原低氧和力竭运动对大鼠骨骼肌磷酸化AMP激活的蛋白激酶(phosphorylated AMP-activated pro-tein kinase,pAMPK)水平的影响及其意义。Sprague-Dawley(SD)大鼠随机分为平原组和高原组,高原组大鼠置模拟海拔5000m低压舱中24h,平原组大鼠在舱外饲养。测定大鼠在模拟高原和平原条件下的游泳力竭时间以及力竭运动后骨骼肌糖原、血乳酸含量。Western blot检测骨骼肌组织中pAMPK/AMPK比值。结果显示,急性低氧可显著缩短大鼠游泳力竭时间。运动力竭时,高原组大鼠骨骼肌剩余糖原含量显著高于平原组大鼠,而血乳酸含量显著低于平原组大鼠。无论是在平原还是在模拟高原条件下,运动力竭时,各组大鼠骨骼肌pAMPK/AMPK比值都显著升高,但高原组大鼠骨骼肌pAMPK/AMPK比值明显低于平原组大鼠。以上结果表明,急性高原低氧可显著降低大鼠运动能力,但运动力竭时骨骼肌pAMPK/AMPK比值低于平原条件下水平,提示骨骼肌能量代谢因素可能不是导致进入高原早期运动能力降低的主要因素。
This article aims to investigate the effect of acute altitude hypoxia and exhaustive exercise on the level of phosphorylated AMP-activated pro-tein kinase (pAMPK) in skeletal muscle of rats and its significance. Sprague-Dawley (SD) rats were randomly divided into plain group and high altitude group. Rats in plateau group were set in simulative altitude of 5000m for 24 hours. The time of swimming exhaustion and the content of glycogen and blood lactate of skeletal muscle after exhaustive exercise were measured in rats under simulated plateau and plain conditions. Western blot detection of skeletal muscle tissue pAMPK / AMPK ratio. The results showed that acute hypoxia can significantly shorten swimming time in rats. Exercise exhaustion, plateau group rats skeletal muscle residual glycogen content was significantly higher than the plain group, while the blood lactic acid content was significantly lower than the plain group rats. The pAMPK / AMPK ratio of skeletal muscle of rats in each group increased significantly when exercising exhausted in both plain and simulated plateau, but the pAMPK / AMPK ratio in skeletal muscle of rats in plateau group was significantly lower than that of plain group. The above results indicate that acute altitude hypoxia can significantly reduce the motor ability of rats, but the exercise-induced exhaustion of skeletal muscle pAMPK / AMPK ratio was lower than the level of the plain conditions, suggesting that skeletal muscle energy metabolism may not lead to reduced motor ability to enter the early plateau major factor.