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采用膜分离技术将费氏丙酸杆菌菌体细胞分离,离位合成转化维生素B12,考察了不同类型及孔径的膜对发酵液的处理能力及对菌体细胞活性的损伤情况,分析了不同浓缩条件下维生素B12的转化效果.结果表明,卷式膜比陶瓷膜更适用于发酵液中菌体细胞的分离,用0.22?m卷式膜处理时,细胞的生物活性几乎不受损伤,存活率达99.5%,将发酵液浓缩4倍,中间体Abo-cbi转化率达78.8%,维生素B12产量为53.9 mg/L.
Membrane separation technology was used to separate and isolate cells of Propionibacterium freudenreichii and to synthesize vitamin B12 in vitro. The ability of different types and sizes of membranes to treat the fermentation broth and the damage to cell viability were investigated. The results showed that the membrane was more suitable for the separation of bacterial cells in the fermentation broth than the ceramic membrane.The biological activity of the cells was almost not damaged when treated with 0.22μm membrane and the survival rate Up to 99.5%. The fermentation broth was concentrated 4 times. The conversion rate of Abo-cbi was 78.8% and the yield of vitamin B12 was 53.9 mg / L.