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目的:研究垂体特异性转录因子-1(pituitary-specific transcription factor-1,pit-1)siRNA转染大鼠GH3型垂体瘤细胞后对其生物学特性的影响。方法:设计合成pit-1 siRNA,经脂质体(lipofectin)转染大鼠垂体瘤细胞后,通过RT-PCR检测瘤细胞内pit-1 mRNA表达的变化;制备Boyden小室测定pit-1 siRNA对肿瘤细胞侵袭力和趋化作用的影响。结果:pit-1 siRNA转染大鼠GH3细胞24 h后,pit-1 mRNA和pit-1蛋白的表达明显低于阴性对照组(P<0.05)。透过基质凝胶多孔滤膜的大鼠GH3细胞瘤细胞的数量都显著低于阴性对照组(P<0.05)。结论:pit-1对大鼠垂体瘤细胞的侵袭性呈正相关,在生长激素腺瘤的发病机制中起重要作用。
Objective: To investigate the effect of pituitary-specific transcription factor-1 (pit-1) siRNA on the biological characteristics of GH3-type pituitary tumor cells. Methods: The pit-1 siRNA was designed and synthesized. The pituitary tumor cells were transfected with lipofectin. The expression of pit-1 mRNA in tumor cells was detected by RT-PCR. The expression of pit-1 siRNA in Boyden chamber Effect of Tumor Cell Invasion and Chemotaxis. Results: The expression of pit-1 mRNA and pit-1 protein in pit-1 siRNA transfected GH3 cells was significantly lower than that in the negative control group (P <0.05). The number of rat GH3 cells that passed through the matrix gel porous membrane was significantly lower than that of the negative control group (P <0.05). Conclusion: Pit-1 has a positive correlation with the invasiveness of pituitary tumor cells and plays an important role in the pathogenesis of growth hormone adenoma.