目前,腹腔镜已广泛应用于不孕原因的诊断,但如果缺少卵巢滤泡系统的功能检查,以及对卵细胞成熟性能的了解,单独作为不孕症现代诊断方法,尚感不足。作者对59例经HSG证实为输卵管阻塞,且经过长时期保守治疗无效而考虑采用手术治疗的患者,试图通过腹腔镜检查及卵细胞试管内培养,评价其卵巢滤泡系统的状态及卵细胞试管内受精能力。患者均有规律的双相型月经周期,丈夫的精液正常。镜检前的两个月经周期内,分别于滤泡期早期、排卵前期、黄体期中期测定血清FSH,LH,E_2,PRL及孕酮水平,并与15名具有正常双相月经周期的健康妇女对照。 At present, laparoscopy has been widely used in the diagnosis of infertility, but if the lack of ovarian follicular function tests, as well as the understanding of egg maturity, as a separate diagnosis of infertility as a method, is still not enough. The author of 59 cases of HSG confirmed tubal obstruction, and after a long period of conservative treatment of patients considered to be surgically treated, trying to laparoscopic and in vitro culture of egg cells to evaluate the status of ovarian follicular system and in vitro fertilization of egg cells ability. Patients have regular bipolar menstrual cycle, her husband’s normal semen. Serum levels of FSH, LH, E_2, PRL and progesterone were measured in early follicular phase, preovulatory phase and mid-luteal phase respectively during the two menstrual cycles before the microscopic examination, and were compared with 15 healthy women with normal biphasic menstrual cycle Control.