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目的：探讨男性生殖系感染者精液解脲支原体（ＵＵ） 感染的检测研究。方法：取精液标本检测，以源于解脲支原体尿素酶基因序列为引物，应用聚合酶链反应（ＰＣＲ） 技术对解脲支原体ＤＮＡ 进行扩增。结果：所试几种非解脲支原体培养物均阴性。解脲支原体阳性出现１ 条４１８ ｂｐ 的ＤＮＡ扩增带。３８２４ 例男性生殖道感染者，ＵＵ 阳性检出率２９－１ ％ 。其中急性淋菌性生殖道炎感染率为４８－１ ％ ，淋菌后生殖道炎感染率为３０－２ ％ ，非淋菌性生殖道炎感染率为１３－３％ ，前两者无显著性差异，后者较前两者均有显著性差异，３１ 例生育者ＵＵ 检出率为３－２％ ，４６ 例不育者ＵＵ 检出率为６３ ％ ，两者比较差别显著，其中精子活动不良组感染率为７８ ％ ，形态异常组感染率为７１－４％ ，低密度组感染率６６－７％ ，无精子症组感染率为４０％ 。结论：ＰＣＲ 技术能有效地为临床提供诊断依据。
Objective: To investigate the detection of semen Ureaplasma urealyticum (UU) infection in male genital system. Methods: Semen samples were taken for detection. Ureaplasma urealyticum (Ureaplasma urealyticum) DNA was amplified by polymerase chain reaction (PCR) from Ureaplasma urealyticum urease gene sequence. Results: Several non-Ureaplasma urealyticum cultures tested were negative. U. ureaplasma positive for a 418 bp DNA amplification band. 3824 cases of male genital tract infection, UU positive detection rate of 29-1%. Including acute gonococcal genital tract infection rate of 48-1%, gonococcal genital tract infection rate was 30-2%, non-gonococcal genital tract infection rate was 13-3%, the former two no significant difference, The latter were significantly different from the former two, 31 cases of UU detection rate of 3 to 2%, 46 cases of infertility UU detection rate of 63%, the difference was significant, including sperm motility group The infection rate was 78%, the morbidity rate was 71-4%, the low-density group’s infection rate was 66-7%, and the azoospermia group’s infection rate was 40%. Conclusion: PCR can effectively provide the basis for clinical diagnosis.