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利用从广西某温泉采集的水样分离嗜酸氧化亚铁硫杆菌(Atf菌)和嗜酸氧化硫硫杆菌(Att)并进行纯化,然后将分离纯化后的Atf、Att菌菌株用于低品位磷矿中磷的浸出,并与经过磷矿驯化后的菌株进行浸磷效果对比和分析。研究结果表明:1)Att、Atf菌经过驯化后其生长活性有所提高,Att菌菌液平均pH比原菌有所降低,Atf菌经5~10 g/L磷矿粉驯化后菌液pH降低较明显;2)进行浸矿试验时,驯化菌株对磷矿粉有较强的适应能力,其pH下降较快,且30 d浸出率比原菌均有提高。其中Att菌在有磷培养基和无磷培养基中经过驯化后浸出率均有明显提高,最大提高了近11%;Atf菌在有磷培养基中经2~8 g/L磷矿粉驯化后浸出率提高显著,最大达28%,而在无磷培养基中经过10~15g/L磷矿粉驯化后浸磷效果最优,浸出率比原菌提高了1倍。
An Atferra acidithiobacillus (Atf) and Attthion acidithiobacillus (Att) were isolated and purified by using a water sample collected from a hot spring in Guangxi, and the isolated and purified Atf, Att strain was used for low grade Phosphate leaching of phosphorus, phosphorus and phosphate after acclimation with strains after phosphorus leaching effect comparison and analysis. The results showed that: 1) Att, Atf bacteria had higher activity after acclimation and the average pH value of Att bacteria was lower than that of the original strain. After Atf bacteria was acclimated by 5 ~ 10 g / L phosphate rock powder, And the decrease was more obvious. 2) During the leaching experiment, the acclimated strains had strong adaptability to phosphate rock and the pH dropped rapidly, and the leaching rate after 30 days was higher than that of the original strain. Among them, Att bacteria grew significantly up to 11% after domestication in both phosphorus and phosphate-free media. Atf was acclimated to 2 ~ 8 g / L phosphate rock in phosphate medium After leaching rate increased significantly up to 28%, while in phosphate-free medium after 10 ~ 15g / L phosphate rock domestication after the best phosphorus leaching rate than the original bacteria increased by 1 times.