论文部分内容阅读
采用ELISA检测68例马来丝虫病人和84例班氏丝虫病人的抗体,发现应用马来丝虫成虫可溶性抗原、马来微丝蚴可溶性抗原和犬恶丝虫成虫可溶性抗原三种抗原测得的阳性检出率无显著性差异.根据测得的消光值和获得材料的难易,认为选用马来丝虫成虫可溶性抗原较好。其敏感性为83.3％～92.6％,特异性为98.8％。分别检测5年和10年前治疗过的马来丝虫病人59例和85例,其ELISA理论转阴率分别为67.1％和82.2％。在一个马来丝虫和三个班氏丝虫病流行区,对四批人群共818名进行了血清流行病学调查,发现ELISA阳性检出率以10岁以下儿童为最低。班氏丝虫病流行区ELISA的阳性检出率与血检微丝蚴阳性率呈平行关系。综上所述,认为ELISA作为丝虫病的辅助诊断方法,对考核与监测丝防工作有实用价值。
Antibody was detected by ELISA in 68 cases of malayian filariasis and 84 cases of bambuterosteal filariasis. It was found that soluble antigens of malayian filarial soluble, soluble antigens of malayian microfilariae and soluble antigens The positive detection rate was no significant difference.According to the measured extinction and the difficulty of obtaining the material, the selection of adult malayian worm soluble antigen is better. The sensitivity was 83.3% ~ 92.6%, specificity was 98.8%. 59 and 85 cases of malayian filariasis treated 5 years and 10 years ago, respectively, the ELISA negative conversion rates were 67.1% and 82.2%. In a Malayan worm and three Bancroftian filariasis endeavors, a total of 818 seroprevalence surveys were conducted among four batches of patients and found that the prevalence of ELISA was lowest among children under 10 years of age. Bancroftian filariasis endemic area ELISA positive rate and the positive rate of blood-screening microfilariae showed a parallel relationship. In summary, ELISA as a diagnostic tool for filariasis, silk screen on the assessment and monitoring work has practical value.