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为探讨结直肠癌患者血清胰腺炎相关蛋白 (PAP)质量浓度在结直肠癌诊断中的作用 ,以及其与肿瘤组织增生活性的关系 ,用ELISA方法测定 2 7例结直肠癌患者和 15名健康人血清PAP质量浓度 ,并将其与癌胚抗原(CEA)和CA1 99值进行比较。通过流式细胞仪测定肿瘤组织DNA倍体性和S期比率 (SPF) ,以此判断肿瘤组织增生活性。结果 :结直肠癌患者血清PAP质量浓度显著高于正常人 (P <0 .0 1)。以血清PAP质量浓度 >2 5μg/L为异常 ,结直肠癌患者高于此值的占 4 4.4 % ( 12 /2 7) ,在这 12例患者中 ,SPF为 17.82± 8.0 2 ,并有 8例非整倍体肿瘤 ,而其余15例PAP正常患者的SPF为 11.17± 7.0 1,且仅有 3例非整倍体肿瘤。两者非整倍体数和SPF的差异有显著性(P <0 .0 5)。在 13例CEA和CA1 99均为阴性的结直肠癌患者中 ,有 3例血清PAP质量浓度升高。结论 :血清PAP水平同CEA和CA1 99联合检测 ,可提高结直肠癌的检出率。血清PAP高于 2 5μg/L的结直肠癌患者 ,其肿瘤组织有较高的增生活性
To investigate the role of serum PAP in colorectal cancer patients in the diagnosis of colorectal cancer and its relationship with the proliferation of tumor tissue, 27 patients with colorectal cancer and 15 healthy persons Human serum PAP mass concentrations were compared with CEA and CAl 99 values. Tumor cell DNA ploidy and S phase ratio (SPF) were determined by flow cytometry to determine tumor tissue proliferative activity. Results: The serum PAP levels in patients with colorectal cancer were significantly higher than those in normal controls (P <0.01). Serum PAP concentration> 25μg / L was abnormal, and the value of colorectal cancer was 4.4% (12/27). In these 12 patients, the SPF was 17.82 ± 8.02 and there were 8 Aneuploidy tumors, while the remaining 15 cases of PAP normal SPF was 11.17 ± 7.0 1, and only 3 aneuploidy tumors. The difference between aneuploidy and SPF was significant (P <0.05). In 13 patients with negative CEA and CA1 99 colorectal cancer, 3 patients had elevated serum PAP concentrations. Conclusion: The combined detection of serum PAP level with CEA and CA1 99 can improve the detection rate of colorectal cancer. Colorectal cancer patients with serum PAP higher than 25μg / L have higher proliferative activity in their tumor tissues