论文部分内容阅读
目的制备异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸(IKVAV)多肽材料并检测其对神经干细胞(NSC)的生物学行为的影响。方法合成IKVAV多肽两亲性分子,进行自组装,用透射电镜(TEM)检测。培养NSC,加入IKVAV,用CCK-8检测吸光度(A值)显示NSC活性。同时检测IKVAV对NSC黏附、分化和轴突生长的影响。结果成功合成IKVAV并自组装成为凝胶,TEM显示为纳米纤维,直径7～8 nm,长度可达到数百纳米。当IKVAV浓度为20 mg/L和60 mg/ L时,A值有明显增加,和对照组相比差异有统计学意义(P＜0．01)。加入IKVAV后,神经球黏附率由4%上升到96%,神经球分化率由6%上升到100%,向神经元分化的比率由4．5%提高到5．6%,同时促进神经轴突生长。结论IKVAV可形成纳米纤维,并且具有良好的神经生物活性,是一种优良的神经生物材料。
Objective To prepare isoleucine - lysine - valine - alanine - valine (IKVAV) polypeptide and test its effect on the biological behavior of neural stem cells (NSC). Methods Amphiphilic molecules of IKVAV peptide were synthesized and self-assembled. The transmission electron microscopy (TEM) was used to detect the IKVAV polypeptide. NSC was cultured, IKVAV was added, and absorbance (A value) was measured by CCK-8 to show NSC activity. The effect of IKVAV on the adhesion, differentiation and axon growth of NSC was also examined. Results IKVAV was successfully synthesized and self-assembled into gel. The TEM showed nanofibers with a diameter of 7-8 nm and a length of several hundred nanometers. When IKVAV concentration was 20 mg / L and 60 mg / L, the value of A increased significantly compared with the control group (P <0.01). After IKVAV was added, the adhesion rate of neurospheres increased from 4% to 96%, the rate of neurosphere differentiation increased from 6% to 100%, and the rate of neuronal differentiation increased from 4.5% to 5.6% Sudden growth. Conclusion IKVAV can form nanofibers and has good neurobiological activity. It is an excellent neurobiological material.