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采用Zn(Ac)2/聚乙二醇600(PEG600)/H2O三元混合溶液作为前驱物,通过100~200℃温度范围内的两阶段加热使Zn(Ac)2水解,再经过高温煅烧使ZnO晶种在SiO2孔壁上形成。以锌氨络合物为锌源,在90℃下热分解后生成的Zn(OH)2沉积在孔道中,并在100℃下利用水热合成原位制备ZnO纳米线,通过改变三元前驱物组分用量以调节ZnO晶种的尺寸与分布,进而控制纳米线的形貌,最终获得了直径为15~20nm的ZnO纳米线,其以无规线团形貌均匀填充于三维孔道中。XRD和拉曼光谱表明纳米线为六方纤锌矿型ZnO晶体。考察了ZnO纳米线/大孔SiO2复合物对猪胰脂肪酶的吸附性能,实验测得复合物的吸附量是单纯大孔SiO2材料的5~6倍,其最大固定量为286.8mg·g-1,最高酶活为425.5U·g-1,被固定的酶蛋白在缓冲溶液中经过48h浸泡不易脱落,且活性保持稳定。
The Zn (Ac) 2 was hydrolyzed by the two-stage heating in the temperature range of 100 ~ 200 ℃ with Zn (Ac) 2 / PEG600 / H2O ternary mixed solution and then calcined at high temperature ZnO seeds are formed on the walls of the SiO2 pores. The Zn (OH) 2 generated after the thermal decomposition at 90 ℃ was deposited in the pores by zinc-ammonia complex as the zinc source, and the ZnO nanowires were prepared in situ by hydrothermal synthesis at 100 ℃. In order to control the size and distribution of ZnO seeds and control the morphology of ZnO nanowires, ZnO nanowires with diameter of 15 ~ 20 nm were obtained. The ZnO nanowires with uniform random coil morphology were filled in three-dimensional pores. XRD and Raman spectra show that the nanowires are hexagonal wurtzite ZnO crystals. The adsorption capacity of ZnO nanowire / macroporous SiO2 complex to porcine pancreatic lipase was investigated. The adsorption capacity of complex was 5-6 times that of pure macroporous SiO2. The maximum immobilized amount was 286.8 mg · g- 1, the highest enzyme activity was 425.5U · g-1, the immobilized enzyme protein was not easy to fall off in the buffer solution after 48h, and the activity remained stable.