论文部分内容阅读
探讨血胰岛素水平对机体碳水化合物反应元件结合蛋白(ChREBP)及乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)表达的影响。分别应用高血胰岛素合并高血糖的KKAy小鼠模型和高血胰岛素DIO小鼠模型,采用Western blotting法观察动物肝脏ChREBP、FAS和ACC蛋白水平。结果显示,KKAy小鼠血胰岛素与血糖水平(r=0.902,P<0.000)、血胰岛素与血TG水平(r=0.732,P<0.000)均呈明显正相关;且随着血胰岛素水平的升高,其肝脏ChREBP、FAS和ACC的蛋白表达也呈渐进性升高。DIO小鼠血胰岛素与血糖水平无明显相关性;血胰岛素与血TG水平呈明显正相关(r=0.722,P<0.001);随着胰岛素水平的升高,肝脏ChREBP及其调控的FAS、ACC的蛋白表达呈渐进性升高。结果提示,在代谢综合征小鼠模型中,血胰岛素水平对肝脏ChREBP的表达具有正向调控作用,机体长期的高血胰岛素状态可能会引起ChREBP及其靶基因蛋白的过表达,从而导致机体的糖脂代谢紊乱。
To investigate the effect of blood insulin on carbohydrate response element binding protein (ChREBP), acetyl CoA carboxylase (ACC) and fatty acid synthase (FAS) expression. The KKAy mice model and hyperinsulinemia DIO mice model with hyperinsulinemia and hyperglycemia were respectively used to observe the protein levels of ChREBP, FAS and ACC in the liver of the animals by Western blotting. The results showed that there was a significant positive correlation between blood insulin level and blood glucose level (r = 0.902, P <0.000), blood insulin level and blood TG level (r = 0.732, P <0.000) in KKAy mice. High, the liver ChREBP, FAS and ACC protein expression also increased progressively. There was no significant correlation between insulin and blood glucose level in DIO mice. There was a positive correlation between insulin and blood TG level (r = 0.722, P <0.001). With the increase of insulin level, hepatic ChREBP and its regulation of FAS, ACC The protein expression was gradually increased. The results suggest that in the mouse model of metabolic syndrome, blood insulin levels have a positive regulatory effect on the expression of ChREBP in the liver. Long-term hyperinsulinemic insulin status may induce overexpression of ChREBP and its target gene proteins, Glucose and lipid metabolism disorders.