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目的：探索诱导ＳＤ大鼠发生实验性变态反应性脑脊髓炎（ＥＡＥ）的可能性，并寻找诱导ＳＤ大鼠ＥＡＥ的合适抗原，以推进国内临床神经免疫学的研究。方法：用含豚鼠、家兔、猪或牛脊髓的完全弗氏佐剂乳化物作为抗原，进行ＳＤ大鼠背部皮肤多点注射，观察和比较动物的神经症状和病理变化。结果：（１）各种抗原诱导ＥＡＥ，如果发病，一般都在注射抗原两周后开始出现症状，在注射后１９天左右症状最明显，且脑组织出现明显的炎性病理变化。（２）注射豚鼠脊髓抗原佐剂乳化物的大鼠发病率为１３％，注射牛脊髓的大鼠发病率为２５％，但注射豚鼠脊髓的大鼠ＥＡＥ症状重于注射牛脊髓的大鼠ＥＡＥ症状；注射兔或猪脊髓抗原佐剂乳化物的大鼠均未发病。结论：诱导ＳＤ大鼠发生ＥＡＥ可能以豚鼠和牛脊髓制备的抗原佐剂乳化物为好，但两者发病率都不高，可能与ＳＤ大鼠对抗原的敏感性较差等因素有关。
OBJECTIVE: To explore the possibility of inducing experimental allergic encephalomyelitis (EAE) in SD rats and find suitable antigens to induce EAE in SD rats to promote the clinical neuroimmunology research in China. Methods: Complete Freund’s adjuvant emulsion containing guinea pig, rabbit, pig or bovine spinal cord was used as antigen to make multi-spot injection on the back of SD rats. The neurological symptoms and pathological changes were observed and compared. Results: (1) EAE was induced by various antigens. Symptoms usually started two weeks after antigen injection. Symptoms were most obvious at 19 days after injection and obvious inflammatory pathological changes occurred in brain tissue. (2) The incidence of rats injected with guinea pig spinal cord adjuvant emulsion was 13% and the incidence of rats injected with bovine spinal cord was 25%. However, EAE in rats injected with guinea pig spinal cord was more severe than those injected with bovine spinal cord Symptoms; rats injected with rabbit or porcine spinal cord antigen adjuvant emulsion were not affected. CONCLUSION: EAE induced by SD rats may be produced by antigenic adjuvant emulsions from guinea pigs and bovine spinal cord. However, the incidence of EAE is not high, which may be related to the poor sensitivity of SD rats to antigens.