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目的:建立高效液相-蒸发光散射检测法测定注射用头孢他啶中碳酸钠的含量。方法:采用Hypersil SCX柱,以0.02mol·L~(-1)醋酸铵溶液(用冰醋酸调节pH至4.8)-乙腈(70:30)为流动相,流速为1.5mL·min~(-1),蒸发光散射检测,检测器温度:40℃,雾化气体(空气)压力:0.35 MPa。结果:Na+在50～501μg·mL~(-1)范围内呈良好的线性关系,r=0.999 4;平均回收率为100.1％,RSD=0.3％,n=6 ; 日内精密度(RSD=1.0％,n=6)良好。结论:本方法简便、快速,结果准确、可靠,重现性好。
Objective: To establish a HPLC-Evaporative light scattering method for the determination of sodium carbonate in ceftazidime for injection. Methods: Hypersil SCX was used as mobile phase with 0.02mol·L -1 ammonium acetate solution (adjusted to pH 4.8 with glacial acetic acid) and acetonitrile (70:30) at a flow rate of 1.5mL · min -1 ), Evaporative light scattering detection, detector temperature: 40 ° C, atomizing gas (air) pressure: 0.35 MPa. RESULTS: Na + showed a good linear relationship in the range of 50 ~ 501μg · mL ~ (-1), r = 0.999 4; the average recovery was 100.1%, RSD = 0.3%, n = 6; %, N = 6) is good. Conclusion: The method is simple, rapid, accurate, reliable and reproducible.