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东北红豆杉(Taxus cuspidata)愈伤组织诱导的最佳培养基为B_5和WH。维持愈伤组织正常快速生长的培养基为B_5。2.4—D对东北红豆杉愈伤组织的诱导和形成起关键作用,其浓度为2mg/L。嫩茎作外植体的愈伤组织诱导率高于针叶。添加适当浓度的有机物会促进愈伤组织的生长速度。
The best culture medium of Taxus cuspidata callus was B_5 and WH. The medium for maintaining normal and rapid callus growth was B_5.2.4-D, which had a key role in callus induction and formation of Taxus cuspidata at a concentration of 2 mg / L. Tender stem explants callus induction rate higher than the needle. Adding appropriate concentrations of organic matter will promote the growth of callus.