检查在Xq27带具有一个脆性部位的标记X染色体fra(X)(q27),已经成为一个常规的细胞遗传学研究,因为它与X-连锁的智力迟钝有着密切关系。检查fra(X)(q27)可以证实对男性患者的诊断,而且对于确定女性携带者也特别有用。在作者实验室里,所有分析都用TC199(含15%的小牛血清)培养外周血的淋巴细胞,分析所有病例的G-显带中期染色体,应用G-显带方法,我们任意地在一个智力迟钝的男性、在许多其他被认为具有明显X-连锁智力迟钝的 Examination of the labeled X chromosome fra (X) (q27) with a fragile site in the Xq27 has become a routine cytogenetic study because of its close relationship with X-linked mental retardation. Checking for fra (X) (q27) can confirm the diagnosis of a male patient and is also particularly useful for identifying female carriers. In the authors’ lab, peripheral blood lymphocytes were cultured in TC199 (containing 15% fetal bovine serum) for all analyzes. G-banding metaphase chromosomes were analyzed in all cases. Using the G-banding method, we randomly selected one Mentally retarded men, in many others, are considered to have significant X-linked mental retardation