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目的：通过胆固醇脂质体（CL）对培养兔胆道口括约肌（SO）细胞作用的研究，以探讨高胆固醇血症与SO功能形态改变间的关系、影响因素及高胆固醇血症诱发胆固醇结石形成的可能机理．方法；分离幼兔胆道口括约肌行原代培养，经免疫组化及电镜鉴定，给予人工CL，分别采用DMEM和RPMI-1640培养基，行细胞增殖四唑盐比色实验（MTT）及流式细胞仪FCM分析．结果：中等浓度（0．5mg／mL～0．8mg／mL）CL对SO细胞有促进增殖作用，表现为S期细胞明显增多，较高浓度（＞1．0mg／mL。）CL则抑制SO细胞生长，表现为非整倍体峰的出现和S期细胞的消失．结论：不同CL浓度可改变SO细胞DNA的复制过程，使SO细胞数量变化，从而表明胆固醇可能通过此机制引起SO功能紊乱而诱发胆系疾病发生．
OBJECTIVE: To study the effect of cholesterol liposomes (CL) on cultured rabbit biliary sphincter (SO) cells in order to investigate the relationship between hypercholesterolemia and the morphological changes of SO, the influencing factors and hypercholesterolemia-induced cholesterol gallstone formation Possible mechanism. Methods: Primary biliary sphincter was isolated from rabbits. The primary CL was isolated and identified by immunohistochemistry and electron microscopy. The cells were cultured in DMEM and RPMI-1640 medium for MTT assay and flow cytometry Cytometer FCM analysis. Results: CL at medium concentration (0.5mg / mL ~ 0.8mg / mL) promoted the proliferation of SO cells, and showed a marked increase of S phase cells. CL at higher concentration (> 1.0mg / mL) inhibited SO Cell growth, the performance of aneuploidy peak emergence and S-phase cells disappear. CONCLUSION: Different concentrations of CL can change the DNA replication in SO cells and change the number of SO cells, which indicates that cholesterol may induce the dysfunction of SO and induce the development of biliary diseases through this mechanism.