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目的对PCR-SSOP方法未明确HLA分型结果的可疑样本进一步分型,并分析原因。方法对采用PCRSSOP方法进行HLA分型出现的131例可疑样本采用LABScan3D分型方法进行检测。对于3D分型仍不能确定的样本采用PCR-SBT分型方法进行检测。结果 131例可疑样本分析,其中87例LABScan3D分型方法可确定分型结果,余下的44例采用PCR-SBT获得分型结果,3种分型方法检测结果在低分水平上是完全一致的。结论 LABScan3D分型方法在PCR-SSOP的基础上进一步发展,可代替其进行大量样本的HLA分型,但是仍有引物探针的局限,不能代替SBT的地位。
OBJECTIVE: To further classify the suspicious samples whose HLA typing results were not confirmed by PCR-SSOP and to analyze the causes. Methods A total of 131 suspected cases of HLA typing by PCRSSOP method were detected by LABScan3D typing method. For the still not sure about the 3D typing, the PCR-SBT typing method was used to detect the samples. RESULTS: A total of 131 suspicious samples were analyzed. Among them 87 cases were classified by LABScan3D method. The remaining 44 cases were genotyped by PCR-SBT. The results of three genotyping methods were completely consistent at the low score level. Conclusion The LABScan3D typing method is further developed on the basis of PCR-SSOP, which can replace HLA typing of a large number of samples. However, there are still some limitations of primer probes and can not replace the status of SBT.