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转氨酶广泛地存在于各组织器官中,神经组织内亦含有丰富的谷草转氨酶,下列的酶促转氨作用很活跃。谷草转氨酶丙氨酸+草酰乙酸(?)α-酮戊二酸+天门冬氨酸至于脑肿瘤组织转氨酶活力的研究结果颇不一致。Green 氏发现良性或恶性脑肿瘤组织的谷草转氨酶活力若按湿重计算,较正常神经组织为高,但若按可溶性蛋白计算,则低于正常。Corridori 氏等则发现,不论根据湿重或可溶性蛋白测算,多数脑肿瘤组织的谷草转氨酶活力是降低的,只有少数高于正常,而 Thompson 氏等认为脑肿瘤组织与正常脑组织的谷草转氨酶活力并无显著差别。为了研究脑肿瘤组织转氨酶活力的变化并观察脑肿瘤组织的转氨作用有无特殊性,我们测定了一些正常脑组织与脑肿瘤组织的转氨酶活力,我们不仅按新鲜组织湿重计算,还同时测定了肿瘤组织的脱氧核糖核酸,由此计算组织的细胞密度,在细胞的水平上来观察酶值的变化。
Aminotransferase widely exists in various tissues and organs, nerve tissue is also rich in aspartate aminotransferase, the following enzymatic transamination is very active. Aspartate aminotransferase alanine + oxaloacetate (?) Α-ketoglutarate + aspartate As for brain tumor tissue activity of aminotransferase quite inconsistent results. Green’s found that aspartate aminotransferase activity in benign or malignant brain tumor tissues was higher than normal nerve tissue if calculated on a wet weight basis but lower than normal if calculated on a soluble protein basis. Corridori et al. Found that the activity of aspartate aminotransferase was decreased in most brain tumors, only a few were higher than normal, regardless of wet or soluble protein, and Thompson et al. Suggested that aspartate aminotransferase activity in brain tumor tissues and normal brain tissues No significant difference. In order to study the changes of transaminase activity in brain tumor tissues and observe the specificity of transamination in brain tumor tissues, we measured the transaminase activity of some normal brain and brain tumor tissues. We not only calculated the wet weight of fresh tissues but also determined The tumor tissues were deoxyribonucleic acid, whereby the cell density of the tissue was calculated and the change of the enzyme value was observed at the cell level.