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[目的]制备高效价特异性鸡卵黄免疫球蛋白(immunoglobulin of yolk,IgY)。[方法]用纯化的旋毛虫幼虫抗原免疫育龄种鸡,经水稀释法初步纯化浓缩后,用ELISA鉴定其免疫学活性,测定纯化后蛋白含量,并用SDS-PAGE进行分析鉴定。[结果]获得ELISA效价为1×106的抗旋毛虫卵黄抗体,蛋白含量为9.67mg/ml。经SDS-PAGE分析,出现2条蛋白带,纯度达到88%以上。[结论]用旋毛虫抗原加弗氏佐剂免疫种鸡制备的卵黄抗体产量大、效价高,这种抗体可进一步用于旋毛虫感染的研究。
[Objective] To prepare high titer specific immunoglobulin of yolk (IgY). [Method] The broiler chickens immunized with purified Trichinella spiralis larvae antigen were purified and concentrated by water dilution method. The immunological activity was identified by ELISA. The protein content was determined by SDS-PAGE. [Result] The anti-Trichinella yolk antibody with ELISA titer of 1 × 106 was obtained with a protein content of 9.67mg / ml. After SDS-PAGE analysis, there are two protein bands, the purity of more than 88%. [Conclusion] The yolk antibody produced by the breeder immunized with Trichinella antigen plus Freund ’s adjuvant has high yield and high potency. This antibody can be further used for the study of Trichinella infection.