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[目的]探讨扁柏醇对肾癌细胞生长、细胞周期和凋亡的影响及其可能作用机制。[方法]以人肾癌786-0细胞为模型,应用MTT法检测扁柏醇对786-0细胞生长抑制,应用流式细胞术检测经扁柏醇处理后786-0细胞周期变化,应用AnnexinV/PI双染法检测扁柏醇诱导肾癌细胞凋亡的发生,用western blot方法分析PARP、JNK蛋白表达和活性的变化。[结果]扁柏醇对786-0细胞的生长有明显抑制作用,呈浓度依赖性和时间依赖性;随着扁柏醇浓度的增加,处于S期的细胞逐渐增加,而G_2/M期的细胞逐渐减少;扁柏醇作用后凋亡细胞也明显增加,并伴随着cleaved-PARP的表达增加;p-JNK蛋白含量随着扁柏醇的浓度增加而升高,而JNK蛋白含量无明显变化。[结论]扁柏醇能明显抑制人肾癌786-0细胞的生长,可能与扁柏醇将细胞阻滞于S期和通过激活SPAK/JNK信号通路诱导细胞凋亡有关。
[Objective] To investigate the effect of hinokitiol on the growth, cell cycle and apoptosis of renal cell carcinoma and its possible mechanism. [Methods] Human renal carcinoma cell line 786-0 was used as a model to detect the inhibitory effect of hinokitiol on the growth of 786-0 cells by MTT assay. The cell cycle of 786-0 cells treated with hinokitiol was detected by flow cytometry. AnnexinV / PI Double staining was used to detect the apoptosis of renal cell carcinoma cells induced by cisplatin. The protein expression and activity of PARP and JNK were analyzed by western blot. [Result] Hinokitiol significantly inhibited the growth of 786-0 cells in a concentration-dependent and time-dependent manner. With the increase of hinokitiol concentration, the cells in S phase increased gradually, while the cells in G2 / M phase gradually Decreased; the apoptotic cells also increased significantly after cisplatin treatment, accompanied by an increase in the expression of cleaved-PARP; p-JNK protein content increased with the concentration of hinokitiol, while the JNK protein content had no significant change. [Conclusion] Janbai alcohol can significantly inhibit the growth of human renal carcinoma 786-0 cells, which may be related to the inhibition of cell growth by cisplatin in S phase and the induction of apoptosis by activating the SPAK / JNK signaling pathway.